A Simple Key For flow cytometer Unveiled
A Simple Key For flow cytometer Unveiled
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Data-enriched characterization of cell populations. Morphological data from photographs can include to the richness of flow cytometry knowledge. By way of example, the figure displays an or else typical apoptosis assay employing Annexin V and PI, adding cell imaging to characterize cells in Each and every populace to expose morphologically distinctive capabilities. These insights couldn't happen to be obtained from multiplex staining by yourself.
As samples are acquired to the Attune CytPix Flow Cytometer, the higher-speed brightfield digicam captures and merchants visuals of detected situations in a amount around 6,000 images per next, depending on the flow price and picture size. For larger overall flexibility, Attune Cytometric Application enables you to regulate the picture capture frequency as desired and select a selected gate for image capture, if required.
(B) RAW 264.7 macrophages will often be annotated inaccurately with regard to particle rely due to their complexity and noncircular borders. The volume of singlets accessible for Evaluation improves as soon as the user trains and employs a far more accurate product.
The award-successful Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing using a large-speed brightfield camera for simultaneous high throughput flow cytometry and substantial resolution brightfield imaging.
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Also, it is illuminating to see the amount of doublets slip by way of classic singlet gates, and fantastic to verify the standing of sudden double-beneficial events in my Examination."
Pair your Attune flow cytometer using an autosampler for greater workflow effectiveness, and add robotic integration for optimum throughput.
Constant impression quality even at higher throughput Acoustic focusing plus a high-speed camera Incorporate to picture these T cells persistently at low or substantial flow fees. Quickly regulate concentration and digital camera settings to fulfill experimental prerequisites.
The Attune NxT cytometer is definitely the trustworthy workhorse instrument your lab can depend upon for reliable and trustworthy performance. The Attune CytPix model features a substantial-pace brightfield digicam and AI-pushed graphic Evaluation software package, with new updates including device Mastering resources and an intuitive user interface, enabling Increased details precision, accuracy, and a lot quicker insights into sample biology.
Maybe most importantly, these functions incorporate cells of clearly distinctive phenotypes which may lead to incorrect conclusions pertaining to double good situations (specifically in uncommon populations).
AI-pushed automatic impression Examination With all the Attune Cytometric Program interprets occasion features into unique morphology parameters that may be combined with common fluorescence and scatter parameters to reveal hugely reproducible populace variations and one of a kind subsets.
We Formerly shown the power of imaging Auto-T/Ramos mobile interactions. Let’s have a look at just the populace of greatest desire, the double beneficial functions, To find out more. We could now use extended morphological parameters (listed here, circularity and skewness of depth) to further examine the characteristics of such populations and refine gating on these events, increasing knowledge robustness.
Leverage the power of State-of-the-art technology and innovation to accelerate your discoveries. Believe in inside the Attune Flow Cytometer spouse and children to deliver the trustworthiness, speed, and precision that set your operate aside.
Adaptable payment plan from Agilent Economical Alternatives help you obtain the vital instrumentation you may need for immediate venture, or to develop your lab.
Gating on these prolonged parameters enables you to immediately and correctly establish populations of curiosity and make sure gating tactic with little or no handbook critique. The Attune program gives usage of point out-of-the-artwork equipment Discovering Investigation equipment in an expertly created user interface, which makes it extremely convenient to use.
The here Attune Xenith cytometer is usually a significant-parameter spectral analyzer, giving the two spectral unmixing and standard flow abilities for detailed and flexible cell Investigation.
Agilent Worth Assure ensures you at the least a decade of instrument use in the day you buy. Normally, We'll credit score you Together with the residual value of the procedure towards an upgraded product.
Below, we exhibit that by gating tactics utilizing graphic-dependent quantitative parameters can distinguish interacting cells from coincident functions to additional properly review interacting cells.